Skip to main content
. 2009 Jan 26;4(1):e4274. doi: 10.1371/journal.pone.0004274

Figure 2. The effect of insulin in IRS-1 phosphorylation with or without pre-exposure of low and high dose testosterone.

Figure 2

(A). The effect of insulin alone on IRS-1 phosphorylation after insulin treatment for 10, 20, 30, 60, and 120 minutes; Insulin significantly increased IRS-1 Ser636/639 phosphorylation after 120 minutes; (B). Cells were pre-exposed to low-dose (20ng/ml) or high-dose (200ng/ml) of testosterone for 16 hours before insulin treatment. Compared to non-testosterone treated (control) cells, IRS-1 Ser636/639 phosphorylation was significantly increased with both low and high-dose testosterone exposure. Representative western blots are shown above the bar graphs. The four experimental groups are: control cells without insulin (C−I), control cells plus insulin (C+I), testosterone exposed cells without insulin (T−I), and testosterone exposed cells plus insulin (T+I). The phosphorylated signal of IRS-1 Ser636/639 is normalized to total IRS-1 in the sample.