Figure 7. TLC demonstrating thioesterase activity of Rv3802 on ¹⁴C-labeled P-CoA and its inhibition by THL, structure above (adapted from DrugBank, http://www.drugbank.ca/drugs/DB01083).

Lanes: 1) ¹⁴C-P-CoA alone, 2–8) serially dilutions decreasing the amount of THL from 12.5–0 µM THL with 3.0 µg of Rv3802. Origin is indicated with an O, start substrate indicated with P-CoA, hydrolysis product indicated with FA (fatty acid).