Results of the SpaA416 ELISA with the 2.5-μg/ml antigen with sera collected from fattening pigs of different ages and sows on two farms. On the first farm, fattening pigs were immunized with live vaccine at age 70 days and sows were immunized every year. On the second farm, fattening pigs were not immunized and only sows were immunized with the live vaccine every year. The hatched columns represent the antibody titers (means and SDs) for sows (n = 15) and fattening pigs at about 30, 50, 70, and 180 days of age (n = 8 at each age) on the first farm; and the white-dotted columns represent the antibody titers (means and SDs) for sows (n = 12) and fattening pigs at about 30, 50, 70, 90, and 180 days of age (n = 8 at each age) on the second farm.