TABLE 1.
Sequences and orientations of the PCR primers used for the cloning of four spaA gene fragments
| Amino acid residuesa | Primer name | Orientationb | Sequence (5′-3′) |
|---|---|---|---|
| 1- | F1 | Forward | 5′-TTTTGGATCCGATTCGACAGATATTTCTGTG-3′ |
| -89 | R89 | Reverse | 5′-TTAAGTCGACGGTGAATGTTTTGAAGACTC-3′ |
| -416 | R416 | Reverse | 5′-TTTTGTCGCTGAATTCGATTCGGGTTTTG-3′ |
| -594 | R594 | Reverse | 5′-CTATGTCGACCTTCCATCGTTCTTAAATGC-3′ |
| 272- | F272 | Forward | 5′-GATAGGATCCAAAGAGGGAAGAAAATACAG-3′ |
| -384 | R384 | Reverse | 5′-TTAAGTCGACAGGCTCTACTTTAGAGTCAC-3′ |
a
The target site is indicated by the positions of the residues corresponding to the amino acid sequence of the mature SpaA of strain Fujisawa.
b
Forward and reverse refer to the orientations of the coding and the complementary strands, respectively.