FIG. 6.
JAK2 and STAT5A mRNA is not inhibited by HIV-1 infection of MDM. Monocytes were cultured for 5 days before infection with HIV-1Ba-L (+HIV) or mock infection (−HIV). MDM were cultured for another 7 days before extraction of mRNA which was used to synthesize cDNA by reverse transcription. (A) PCR was performed on cDNA using primers specific for β-actin, JAK2, and STAT5A. Levels of cDNA were standardized according to β-actin levels. The first sample (undiluted, or neat) of each dilution series corresponds to 20,000 MDM. (B) The supernatant was removed from each culture of MDM (on day 12 after isolation of the cells), and the level of HIV-1 infection was assessed by RT assay. Results for STAT5A PCR are representative of MDM prepared from four donors, and results for JAK2 PCR are representative of MDM prepared from two donors.