FIG. 1.

Immunoblot analysis of CD45-depleted microvesicles produced from CEMx174 T1. (A) Analysis of fractions from CD45 depletion of 50 μg (total protein) of microvesicles is shown. Samples are identified above their respective lanes as follows: starting material, S; flowthrough, F; wash, W; and bead, B. The antibody or antiserum used for each blot is indicated at the right. (B) Analysis of the CD45 content of starting (S) and flowthrough samples from a series of depletions using various ratios of CD45 microbeads (microliters) to microvesicle protein (micrograms) is presented. The ratio used for each depletion is indicated above its respective lane. Molecular mass standards are indicated at the left.