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HPLC Analysis of CD45-depleted HIV and SIV. A280 chromatograms of HPLC separations of untreated and treated HIV-1 and SIV samples are presented. Absorbance levels of the chromatograms were normalized to the NC peak height to allow for the assessment of the relative purity of the virus preparations in the starting (−) and CD45-depleted (+) material. Important peaks identified by immunoblot or protein sequence are labeled above their respective chromatograms.
