FIG. 4.

Mammalian cell-based two-hybrid reporter assay reveals that mutants M5, M10, and M14 fail to efficiently interact or multimerize in vivo. 293T cells were cotransfected with 0.4 μg of Gal4 chimeric expression vector, 0.4 μg of VP16 chimeric expression vector, and 0.2 μg of the luciferase reporter plasmid pG₅Luc. At 48 h posttransfection, cells were lysed and tested for luciferase activity. The average luciferase values for three independent experiments are presented as the change in induction (n-fold) over Gal4Rex-2 alone (set at 1).