TABLE 2.

Summary of Rex-2 mutant proteins

Rex-2 proteins	Localizationa	RNA bindingb	Multimerization/trans dominantc	Shuttlingd
wtRex-2	Nuc	+	+/−	+
Rex-2 M1	Nuc	+	+/−	+
Rex-2 M5	Nuc	+	−/+	±
Rex-2 M10	Nuc	+	−/+	±
Rex-2 M14	Nuc	+	−/+	±
Rex-2 M16	Nuc	+	+/−	±
Rex-2 M17	Cyt	±	NDe/−	NDe
Rex-2 DD	Nuc	++	+/NDe	±
Rex-2 M18	Nuc	+	+/−	+
Rex-2 M19	Nuc	+	+/−	±

^aPredominant subcellular localization. Nuc, nucleus and nucleolus; Cyt, cytoplasmic.

^bPositive (+), impaired (±), or enhanced (++) Rex binding to RxRE-containing RNA as determined by in vivo binding experiments.

^cPositive (+) or negative (−) for multimerization and trans-dominant capacity.

^dPositive (+) or impaired (±) for nucleocytoplasmic shuttling as determined by actinomycin D assays.

^eND, not determined.