FIG. 4.

Spleen memory CD8⁺ T cells from pCCR7L-treated mice secreted IFN-γ upon restimulation ex vivo more rapidly than CD8⁺ T cells from non-pCCR7L-treated mice. The cells were isolated at 60 days postboost and assayed for IFN-γ production ex vivo by intracellular cytokine staining. Spleen cells (10⁶) were incubated in the presence of 2.5 μg of HSV-gB_498-505, 50 U of IL-2, and GolgiPlug for 5 h and subsequently stained with anti-CD8⁺-FITC and anti-IFN-γ-phycoerythrin (PE) antibodies (except for groups treated with UV-inactivated HSV and PBS, for which IFN-γ-FITC and CD8⁺-PE were used). FITC-conjugated rat anti-IgG was used for the isotype control (data not shown). Cytometry and data analysis were performed with FACScan and Cell Quest, respectively. Figures show representative data from two independent experiments.