TABLE 1.

pCCR7L codelivery with rVVgB and pgB influences serum and genital tract wash fluid antibody levels^a

Group	Response (ng/ml)
	Primary in:				Memory in:
	Serum		Vaginal tract wash fluid		Serum		Vaginal tract wash fluid
	IgA	IgG	IgA	IgG	IgA	IgG	IgA	IgG
rVVgB + pgB	47 ± 12	29,138 ± 1,002	24 ± 2	1,869 ± 213	15 ± 7	3,896 ± 256	NDb	569 ± 49
pCCL21 + rVVgB + pgB	60 ± 8	48,883 ± 1,201†	57 ± 12	2,188 ± 121	21 ± 9	5,986 ± 564	ND	745 ± 213
pCCL19 + rVVgB + pgB	58 ± 6	54,765 ± 7,753††	87 ± 16	2,371 ± 446	22 ± 8	5,265 ± 143	ND	629 ± 146
pβ-Galc	19 ± 1	103 ± 11	13 ± 5	24 ± 21	0	0	ND	0
UV-HSVd	201 ± 23	73,872 ± 14,036†††	146 ± 18	2,972 ± 602	34 ± 6	13,003 ± 123	ND	1,092 ± 97
PBS	0	28 ± 19	0	29 ± 18	0	0	ND	0

^aMice were immunized with rVVgB and pgB with or without codelivery of pCCR7L. Serum samples and genital tract wash fluid were collected at 14 days after boost (primary response) and 60 days after boost (memory response). ELISA for antibody detection was performed as described in Materials and Methods. Data represent means ± standard deviations for four animals per group in one experiment from two performed. †, P ≤ 0.05 in comparison to rVVgB-, β-Gal-, and PBS-treated mice; ††, P ≤ 0.05 in comparison to rVVgB-, β-Gal-, and PBS-treated mice; †††, P ≤ 0.05 in comparison to all groups.

^bND, not detected.

^cpβ-Gal, plasmid DNA encoding β-Gal.

^dUV-HSV, UV-inactivated HSV.