FIG. 2.

Mutation in the sequence coding for PV protein 3A causing defects in RNA replication. (A) The amino acid sequence of the C-terminal part of wild-type PV 3A protein is shown with the residue numbers in 3A indicated above it. The cleavage site between 3A and 3B is indicated after amino acid 87. The hydrophobic domain of protein 3A is underlined. Residue methionine 79 is circled. The codon for methionine and the changes introduced by site-directed mutagenesis are shown. (B) Time course of RNA replication measured by luciferase accumulation following RNA transfection of HeLa cells at 37°C.