TABLE 1.

Oligonucleotides used for mutagenesis

Oligonucleotide	Sequence (5′-3′)a
3AM79V frwd	GGAGTTGTCTATGTCGTGTATAAACTGTTTGCTGG
3AM79V rev	CCAGCAAACAGTTTATACACGACATAGACAACTCC
3AM79T frwd	GTTGTCTATGTCACGTATAAACTGTTTGC
3AM79T rev	GCAAACAGTTTATACGTGACATAGACAAC
3AM79L frwd	GTTGTCTATGTCCTGTATAAACTGTTTGC
3AM79L rev	GCAAACAGTTTATACAGGACATAGACAAC
3AH86Y frwd	GTTTGCTGGATATCAGGGAGCATACAC
3AH86Y rev	GCTCCCTGATATCCAGCAAACAG
3AM79VH86Y frwd	GGAGTTGTCTATGTCGTGTATAAACTGTTTGCTGGATACCAGGGAGCATACACTGG
3AM79VH86Y rev	CCAGTGTATGCTCCCTGGTATCCAGCAAACAGTTTATACACGACATAGACAACTCC
3DK276L frwd	CACCTGTACCTGAATAAAACATACTGTGTCAAGGG
3DK276L rev	CACAGTATGTTTTATTCAGGTACAGGTGGTGTGAG

^aSubstituted nucleotides are indicated in bold and underlined.