FIG. 2.

p107 overexpression inhibited histone H1 phosphorylation catalyzed by cyclin E/cdks, and IE1-72 expression reversed this inhibitory effect. (A) Western blot analyses demonstrating expression of p107, IE1-72, and IE2-86 in C33A cells transfected with the indicated constructs. (B) Kinase assays of complexes immunoprecipitated with anti-cyclin E antibody from lysates of mock-transfected cells (lane 1), cells transfected with p107 plasmid alone (lane 2), cells cotransfected with p107 plasmid and increasing amounts of IE1-72 plasmid (lanes 3 to 5), or cells cotransfected with p107 plasmid and IE2-86 plasmid (lane 7).