FIG. 2.

Expression of viral proteins. RK13 cells were infected at a MOI of 2 with PrV-Ka, PrV-ΔUL46-49, PrV-UL47R, PrV-UL48R, or PrV-UL46-49R and incubated for 16 h at 37°C. Proteins were separated in discontinuous sodium dodecyl sulfate-polyacrylamide gels and transferred to nitrocellulose filters. Western blots of infected and noninfected cells were probed with a monoclonal antibody against gB (α-gB) or monospecific antisera against viral tegument proteins (α-UL46, α-UL47, α-UL48, and α-UL49) and gN, encoded by the UL49.5 gene (α-gN). Detected proteins were visualized by chemiluminescence reactions (Super Signal; Pierce) of peroxidase-conjugated secondary antibodies (Dianova). Molecular masses of marker proteins are indicated at the left.