FIG. 9.

Inhibition of MHV-68 virion production by NS-398 is reversed by PGE₂. NIH 3T3 cells were pretreated with DMSO (D) or NS-398 (0.5 or 5 μM). Subsequently, cells were infected with wt MHV-68 and maintained in the appropriate DMSO-NS-398 concentrations. Immediately after infection, NS-398-treated cells were also treated with PGE₂ (0.1 or 1 μM). Supernatants were harvested at 12 h postinfection, and viral titer was quantitated using plaque assays on BHK-21 monolayers.