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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Cell Calcium. 2008 Jul 11;45(1):29–37. doi: 10.1016/j.ceca.2008.05.006

Figure 2.

Figure 2

Efficacies of knocking down junctin and triadin in C2C12 myotubes. Eight-day-old control (A and C) or double knockdown (B and D) myotubes were probed with rabbit anti-junctin polyclonal antibody (A and B) or mouse anti-triadin monoclonal antibody (C and D) followed by the corresponding Alexa 647-conjugated goat-anti-rabbit or Alexa 488-conjugated goat-anti-mouse secondary antibody. The efficacies of the triadin siRNA and junctin siRNA are indicated by the nearly complete elimination of fluorescence in the double knockdown myotubes. Insets in B and D show pictures of corresponding double knockdown myotubes. Scale bars in (A) and (C): 10 μm.