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Effects of junctin and triadin knockdown on stored Ca2+ release in 8-day-old C2C12 myotubes. Depolarization (10 mM KCl) (A), caffeine (10 mM) (B), and UTP (400 μM) (C) induced Ca2+ release were determined in Ca2+-free KRH bath solutions as changes of Fluo-4 fluorescence (F/F0) in C2C12 myotubes infected with rAAV-control (top traces), rAAV-triadin (second traces), rAAV-junctin (third traces), and rAAV-junctin/triadin (bottom traces).
