Table 1.

Effects of junctin/triadin knockdown on stored Ca²⁺ release in C₂C₁₂ myotubes

Addition	Control	Junctin knockdown	Triadin knockdown	Junctin/triadin knockdown
10 mM KCl	1.98 ± 0.25	1.61 ± 0.18*	1.49 ± 0.13*	1.27 ± 0.08*
0.4 mM 4-chloro-m-cresol	1.88 ± 0.23	1.65 ± 0.17*	2.05 ± 0.14	1.60 ± 0.11*
10 mM caffeine	1.83 ± 0.17	1.65 ± 0.14*	1.88 ± 0.24	1.69 ± 0.18*
1 μM thapsigargin	1.86 ± 0.17	1.64 ± 0.16*	1.93 ± 0.24	1.63 ± 0.14*
0.4 mM UTP	2.07 ± 0.29	1.46 ± 0.08*	1.90 ± 0.16	1.48 ± 0.07*

Peak values of fluorescence increases (F/F₀) in C₂C₁₂ myotubes were determined in Fluo-4 loaded cells in Ca²⁺-free KRH buffer. Data are the mean ± S.E. of 8–17 experiments.

^*p < 0.05 compared to control.