Table 2.
Quantitative RT-PCR of mRNAs from junctin and triadin knockdown myotubes.
| MRNA | Control | Junctin knockdown | Triadin knockdown | Junctin/triadin knockdown |
|---|---|---|---|---|
| RyR1 | 100 | 131 ± 14 | 99 ± 7 | 103 ± 6 |
| RyR3 | 100 | 126 ± 12 | 109 ± 11 | 124 ± 13 |
| DHPR-α1 | 100 | 82 ± 23 | 107 ± 26 | 72 ± 22 |
| CSQ1 | 100 | 137 ± 29 | 68 ± 26 | 76 ± 23 |
| CSQ2 | 100 | 130 ± 22 | 105 ± 11 | 116 ± 16 |
| SERCA1 | 100 | 109 ± 10 | 97 ± 31 | 94 ± 19 |
RT-PCR analyses were performed using total RNA isolated from 8-day-old myotubes. Relative levels of gene expression as a percentage of control were determined for each gene with β-actin as reference. Data are the mean ± S.E. of 3–12 samples.