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. Author manuscript; available in PMC: 2009 Sep 30.
Published in final edited form as: Virology. 2008 Aug 5;379(2):234–244. doi: 10.1016/j.virol.2008.06.043

Fig. 2. Expression of LANA adjacent genes following genetic deletion of LANA from the KSHV genome.

Fig. 2

(A) Reconstitution of recombinant viruses in HEK293 cells by transfection. Images were taken at 48 h post-transfection. (B) Expression of LANA, vCyclin and vFLIP transcripts in BAC36, BAC36ΔLANA and BAC36ΔLANArt cells analyzed by RT-PCR. GAPDH was used as a loading control. (C) Expression of LANA and vCyclin proteins in BAC36, BAC36ΔLANA and BAC36ΔLANArt cells examined by IFA using specific antibodies.