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. Author manuscript; available in PMC: 2009 Jan 14.
Published in final edited form as: J Immunol. 2007 Apr 1;178(7):4498–4505. doi: 10.4049/jimmunol.178.7.4498

Figure 5.

Figure 5

IFN-γ production by CD8 and CD4 T cells day 8 after infection with 106 Lm-OVA ΔactA in B6 mice, IFN-IR−/− mice, IL12P40−/−, IL12P35−/−, or IFN-IR−/−IL12P40−/− mice. FACS plots demonstrating percent (A) and total number (B,C) of IFN-γ producing CD8 T cells per spleen after stimulation with the MHC class I peptides: OVA257−264, LLO296−304, or no peptide control. FACS plots demonstrating percent (D) and total number (E) of IFN-γ producing CD4 T cells per spleen after stimulation with LLO189−201 peptide or no peptide. G. Concentration of IFN-γ in splenocytes culture supernatants 72 hours after stimulation with OVA257−264 peptide (MHC class I), LLO189−201 peptide (MHC class II), or no peptide as determined by ELISA. Each data point represents 4−6 mice per experimental group from two independent experiments. Bar, one standard error.