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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: Dev Biol. 2008 Aug 7;322(2):322–331. doi: 10.1016/j.ydbio.2008.07.035

Figure 5.

Figure 5

A yeast two-hybrid assay with AD-SKR-1 or AD-SKR-1(M140) and DB-CUL-1 or empty vectors (−).Yeast were plated in a 0.5x dilution series from left to right starting at OD600 = 10. Positive interactions exhibit increased growth on 3AT relative to negative controls (lanes 1, 2, and 5). ®-galactosidase activity based on CPRG assay is shown as a percentage of the activity observed in yeast expressing SKR-1(+)/CUL-1(+). Yeast were grown for two days before imaging. See Materials and Methods for additional details.