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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: Dev Biol. 2008 Aug 7;322(2):322–331. doi: 10.1016/j.ydbio.2008.07.035

Table 1.

Reduction of the skr-1 activity suppresses sel-10(n1074) masculinization phenotypes

genotype % CEM % HSN % Male
B cell
morphology		 % Male
coelomocyte
position
+ 0 100 0 0
+ male 100 0 100 100
sel-10(n1074) 86 1 92 64
skr-1(sm151); sel-10(n1074) 14 38 50 11
skr-1(sm151) 0 100 0 0
skr-1(sm151) male3 100 0 ND ND
skr-1(sm151)/+; sel-10(n1074)4 62 3 ND ND
skr-1(tm2391)/+; sel-10(n1074)5 43 8 ND ND
skr-1(sm151)/skr-1(tm2391); sel-10(n1074)6 1 77 ND ND

All strains have XX karyotype, unless otherwise indicated (XO male), and carry smIs26, which harbors Ppkd-2gfp, Ptph-1gfp, and unc-76 rescuing plasmid. n = 400 for CEMs, 100 for HSNs, and 50 for both B cell and coelomocyte scoring. Scoring of CEMs, HSNs, B cells and coelomocytes is described in Materials and Methods. ND = not determined.

1

Male B cell morphology: B cells in L1 larvae with enlarged nucleoli.

2

Male coelomocyte position: one left ventral coelomocyte located posterior to the L1 somatic gonad primordium.

3

Actual genotype skr-1(sm151); smIs26; him-5(e1490)

4

Actual genotype skr-1(sm151)/dpy-5(e61) unc-29(e193); smIs26; sel-10(n1074).

5

Actual genotype skr-1(tm2391)/dpy-5(e61) unc-29(e193); smIs26; sel-10(n1074).

6

Actual genotype skr-1(tm2391)/dpy-5(e61) skr-1(sm151); smIs26; sel-10(n1074).