PKCβII preferentially translocates to cell–cell junctions. A, 3D images showing subcellular distribution of PKCβII relative to VE-cadherin in nonactivated and PDBu-activated HCMECs (image depth=10.15μm). B, PKCβII density (labeled pixels) at the junction and cytosol before and after activation. C, Subcellular colocalization between PKCβII and VE-cadherin (B and C, n=8, *P<0.05).