Comparative Analysis of the Multiple hGR Gene Promoter GRUs
A, Comparison of the long sequences for the hGR 1A promoter (−981/+269), the sequence that contains the two most 5′-GRUs in the proximal GR promoter (−5144/−3608), and the sequence that contains the most 3′ two GRUs in the proximal GRU promoter (−3850/−2523), and the effect of PU.1 overexpression in Jurkat cells. B, Comparison of the minimal GRUs for the hGR 1A promoter (+243/+269), the strongest 5′-upstream GRU (1D?; −4559/−4525) in the proximal GR promoter, and the strongest 3′-GRU (1C?; −2956/−2916) in the proximal GRU promoter, and the effect of PU.1 overexpression in Jurkat cells. The results are displayed as DEX induced percent over the EtOH control. *, P < 0.05; **, P < 0.01; ***, P < 0.005.