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. 2009 Jan 19;4(1):e4221. doi: 10.1371/journal.pone.0004221

Figure 3. Spatial Expression Pattern of Validated Proteins in in vivo Embryonic Hearts.

Figure 3

Immunofluorescence (red or green) was performed on hearts excised at E9.5 to confirm that the validated proteins identified in the vascular dataset are also present in the heart (A–E, G–O). Insert contains the DAPI (blue) merged image. F: Whole mount β-gal staining of an E9.5 NOGO-A/B-lacZ heart. Outflow Tract (OFT); Ventricle (V); AVC (Atrioventricular Canal); A (Atrium); Myocardium (Myo); Endocardium (En); Mesenchyme (Mes); Scale bar = 50 µm.