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. 2009 Jan 21;4(1):e4232. doi: 10.1371/journal.pone.0004232

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Capital letters represent tra genes and lower case letters represent trb genes. Black arrows represent known ORFs genes, grey arrows represent truncated genes, and white arrows represent hypothetical protein genes. PCR of the traI-traB region on 0.8% agarose gel (C), (L) 10 kb ladder (Promega), (1) χ7122, (2) χ7346, (3) χ6092.