Table 1. Bacterial strains and plasmids used in this study.
Strain/ plasmid | Relevant characteristics* | Reference or source |
Strains | ||
MGN-617 | SM10 λpir derivative, thi thr leu tonA lacY supE λpir recA::RP4-2-Tc::Mu (Kmr) ΔasdA1 | [33] |
TOP10 | E. coli K-12, Lac− F− Strr | Invitrogen |
χ833 | E. coli K-12, Lac− F− Strr | This study |
χ2934 | E. coli K-12, Lac− F− Nalr | This study |
χ6092 | E. coli K-12, Lac− F− Tcr | This study |
χ7122 | APEC O78∶K80∶H9, gyrA Nalr | [22] |
χ7273 | χ7122 tsh::tetAR(B), Nalr Tcr | [16] |
χ7276 | E. coli K-12 MG1655 Tn10::kan, Kmr | [16] |
χ7277 | χ7276 pAPEC-1-1 pAPEC-2, Kmr Tcr | [16] |
χ7345 | χ2934 pAPEC-1-1 Nalr Tcr | This study |
χ7346 | χ6092 pAPEC-1 Tcr | This study |
χ7396 | MGN-617 pAPEC-1-1, Kmr Tcr Asd− | This study |
39R681 | E. coli containing four plasmids 147 kb, 63 kb, 35.85 kb, and 6.9 kb | [83] |
Plasmids | ||
pAPEC-1 | Virulence plasmid of APEC χ7122 | [16] |
pAPEC-1-1 | pAPEC-1 tsh::tetAR | [16] |
Km, kanamycin; Nal, nalidixic acid; Str, streptomycin; Tc, tetracyclin.