Table 2. Functional redundant activities between Nkx2.5 and Nkx2.7 in zebrafish heart development.

Morpholino (ng)		mRNA (pg)		Total (n)	Heart Defects (%)
Nkx2.5	Nkx2.7	Nkx2.5	Nkx2.7
0	9 ng	0	0	177	55%
0	9 ng	10 pg	0	109	32%
0	9 ng	20 pg	0	113	22%
8 ng	8 ng	0	0	146	91%
8 ng	8 ng	25 pg	0	278	73%
8 ng	8 ng	50 pg	0	169	58%
8 ng	8 ng	0	0	166	92%
8 ng	8 ng	0	0.25 pg	189	61%
8 ng	8 ng	0	0.5 pg	206	43%

For rescue study, the Nkx2.5 mRNA was co-injected with Nkx2.7-MO into embryos derived from zebrafish transgenic line Tg(cmlc2::GFP). The percentages of heart defects were decreased compared to phenotypes which occurred in the embryos injected with Nkx2.7-MO alone. Similarly, either the Nkx2.5 or Nkx2.7 mRNA enabled embryos to be rescued from the defects as a result of the injection of Nkx2.5/2.7-MO. n: total number of embryos analyzed.