Figure 2. UBP2 and RUP1 interact genetically with RSP5.

(A, B, D) The indicated over-expression plasmids, driven by a galactose inducible promoter, were transformed into either wildtype or mutant strains. Cell cultures were then serially diluted 10-fold (starting OD₆₀₀ of 0.5) and spotted onto SC-URA media containing glucose (non-inducing) or galactose (inducing). (B) Growth of rsp5-326 mutant cells bearing pHA-RSP5 (a low copy, but not galactose responsive plasmid), and/or a second overexpression plasmid as indicated. (C) Growth of cells deleted for either UBP2 and RUP1 alone, or in combination with an RSP5 temperature sensitive allele (rsp5-1), on rich media (YPD) at either 30°c (permissive for rsp5-1) or 34°c (semi-permissive for rsp5-1).