Figure 3. Loss of UBP2 results in hypersensitivity to 5-fluorouracil (5-FU).

Wildtype, ubp2Δ, vps37Δ, fur4Δ single mutants, and vps37Δubp2Δ double mutant cells were transformed with a URA3 plasmid and subsequently tested for sensitivity to 5-FU. Exponentially growing cells were serially diluted 10-fold (starting OD₆₀₀ ∼0.7) and spotted onto SD plates with 5 µM 5-FU. Plates were imaged either after 4 days (left panel) or 7 days (right panel).