Figure 8. ubp2Δ cells are sensitive to alpha-factor consistent with a defect in Ste2 receptor sorting.

α-factor (5 ul of 100 µM, 40 µM or water alone) was spotted onto sterile filter disks, and placed onto solid media containing WT, ubp2Δ, rup1Δ, or end3Δ MATa cells. Plates were then incubated at 30°C for one day and imaged. Halo diameters (mm) were measured and averaged from five replicates of 100 µM alpha factor spots.