Table 1. Yeast Strains Used in this Study.

Strain/Plasmid	Relevant Genotype	Origin
WT	BY4741 (MATa his3Δ1 leu2Δ0 ura3Δ0 met15Δ0)	[68]
Ubp2-TAP	MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 UBP2::TAP-HIS3	[69]
Rup1-TAP	MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 RUP1::TAP-HIS3	[69]
ubp2 Δ	ubp2Δ::NATMX, derived from Y3656 (MATα can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 his3Δ leu2Δ0 ura3Δ0 met15Δ0 lys2Δ0)	This study, [70]
ubp2 Δ	MATa his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 ubp2Δ::KanMX3	[25]
rup1 Δ	rup1Δ::NATMX, derived from Y3656 (MATα can1Δ::MFA1pr-HIS3-MFα1pr-LEU2 his3Δ leu2Δ0 ura3Δ0 met15Δ0 lys2Δ0)	This study, [70]
rup1 Δ	MATa his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 rup1Δ::KanMX3	[25]
rup1 Δ	rup1Δ::NATMX, derived from FY56 (MATα his4-912 ΔR5 lys2-128Δ ura3-52)	This study, [71]
Ubp2-TAP rup1 Δ	MATa UBP2::TAP-HIS3, rup1Δ::NATMX	This study
Rup1-TAP ubp2 Δ	MATa RUP1::TAP-HIS3, ubp2Δ::NATMX	This study
rsp5-1	FW1808 (MATα his4-912 ΔR5 lys2-128Δ ura3-52 rsp5-1)	[71]
rsp5-326	MATa leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 ade2-101 suc2-Δ9	[35]
ubp2 Δ rsp5-1	ubp2Δ::KanMX3 rsp5-1	This study, [71]
rup1 Δ rsp5-1	rup1Δ::KanMX3 rsp5-1	This study, [71]
fur4 Δ	MATa his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 fur4Δ::KanMX3	[25]
end3 Δ	MATa his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 end3Δ::KanMX3	[25]
vps37 Δ	vps37::HIS	This study
vps37 Δ ubp2 Δ	vps37::HIS ubp2Δ::KanMX3	This study