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. 2008 Dec;173(6):1714–1723. doi: 10.2353/ajpath.2008.080622

Figure 5.

Figure 5

A role of Vα14 iNKT cells in the regulation of mesenteric Th17 cells. A: IL-17 production by the MLN T cells of mice lacking invariant iNKT or MAIT cells. After 1 week of KCV treatment, MLN cells were isolated from control or KCV-treated mice, including wild-type (WT), Mr1−/−, β2m−/−, Cd1−/−, or Jα281−/− mice. The cells were stimulated with immobilized anti-CD3 antibody for 72 hours. IL-17 in the supernatant was measured using ELISA. Data are a representative of two independent experiments (n = 2 mice). *P < 0.05 (Student’s t-test). B: Th17 cells in MLNs in KCV-treated or iNKT deficient mice. MLN cells were isolated from wild-type mice (WT control), KCV-treated wild-type mice (WT KCV), or iNKT cell-deficient Jα281−/− mice and stimulated for 5 hours with phorbol 12-myristate 13-acetate and ionomycin in the presence of GolgiPlug. We conducted surface labeling with the indicated antibody and αGalCer-loaded CD1 days dimer as well as intracellular IL-17 staining. Dot plots are gated on CD4+ T cells devoid of iNKT cells. Data are representative of two independent experiments (n = 2 mice).