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. 2008 Dec;173(6):1758–1767. doi: 10.2353/ajpath.2008.080363

Figure 3.

Figure 3

Interaction of AR and MCM7 leading to increase or decrease of MCM7-chromatin association. A: Immunofluorescence staining of MCM7 in PC3 cells transfected with pCMV-AR, or PC3 cells transfected with mutant AR, or LNCaP cells, untreated or treated with a low dosage or a high dosage of R1881. B: Chromatin association of MCM7 and AR induced by R1881. Chromatin extracts form LNCaP, PC3+AR, and PC3+ΔAR cells treated with indicated dosage of R1881 for 0.5, 24, and 48 hours. The chromatin associations of AR or MCM7 or nonchromatin MCM7 were visualized on immunoblots with antibodies against AR or MCM7. Antibodies against histone 3 were used as normalization controls. C: Densitometry analysis of the ratios of chromatin/nonchromatin MCM7 of B.