Table 1.

Primers for Generating pGST-MCM7, pGST-AR, and pET-MCM7n Constructs

Primer names	Sequence
MCM7n1a:	5′-TGGGATCCCACTGAAGGACTACGCGCTAGAG-3′
	5′-CGCTCGAGATTTCCCACAGGCACCTGATCACT-3′
MCM7n2a:	5′-GGGGATCCCCGAGTTGGTGGACTCAATTTGT-3′
	5′-CGCTCGAGATTTCCCACAGGCACCTGATCACT-3′
MCM7n3a:	5′-AGGGATCCTGGACGTTTACATTGAGCATCGG-3′
	5′-CGCTCGAGATTTCCCACAGGCACCTGATCACT-3′
MCM7n4a:	5′-CCGGATCCCTGCTGAACTCATGCGCAGATTT-3′
	5′-CGCTCGAGATTTCCCACAGGCACCTGATCACT-3′
MCM7n5a:	5′-AAGGATCCGTGGAATCGTCACTCGTGTCTCT-3′
	5′-CGCTCGAGATTTCCCACAGGCACCTGATCACT-3′
MCM7n6a:	5′-TTGGATCCCTCTGATCATGTGCCCAAGCCAG-3′
	5′-CGCTCGAGATTTCCCACAGGCACCTGATCACT-3′
MCM7n7a:	5′-ATCTGCGGATCCGGGGCTCCAGATTCATC-3′
	5′-CGCTCGAGATTTCCCACAGGCACCTGATCACT-3′
ARI	5′-ATCGAAGGTCGTGGGATCCAAGTGCAGTTAGGGCTGGGAAGG-3′
	5′-CCTTGAAAGGGGAATACTCGAGCAGTATCTTCAGTGCTCTTG-3′
ARII	5′-ATCGAAGGTCGTGGGATCCCTGCTGAGTATTCCCCTTTCAAG-3′
	5′-CTTCCGGGCTCCTCGAGTCATCCCTGCTTCATAACATTTCCG-3′
ARIII	5′-ATCGAAGGTCGTGGGATCCCTCTGGGAGCCCGGAAGCTGAAG-3′
	5′-ACATCTGAAAGGGGGCTCGAGCTGGGGTGGGGAAATAGG-3′
ARII1b:	5′-ATCGAAGGTCGTGGGATCCCTGGCGGCATGGTGAGCAGAGT-3′
	5′-CTTCCGGGCTCCTCGAGTCATCCCTGCTTCATAACATTTCCG-3′
ARII2b:	5′-TAATAGGATCCCGGGAGCTGTAGCCCCCTACGGCTAC-3′
	5′-CTTCCGGGCTCCTCGAGTCATCCCTGCTTCATAACATTTCCG-3′
ARII3b:	5′-AGTTGTATGGATCCCGTGTGGTGGTGGTGGGGGTGG-3′
	5′-CTTCCGGGCTCCTCGAGTCATCCCTGCTTCATAACATTTCCG-3′
ARII4b:	5′-GTTCTGGGATCCCCTCAGCCGCCGCTTCCTCATCC-3′
	5′-CTTCCGGGCTCCTCGAGTCATCCCTGCTTCATAACATTTCCG-3′
ARII5b:	5′-ATAACCCGCTGGATCCACGGCAGCGCCTGGGCGGCTG-3′
	5′-CTTCCGGGCTCCTCGAGTCATCCCTGCTTCATAACATTTCCG-3′
ARII1a:	5′-ATCGAAGGTCGTGGGATCCCTGCTGAGTATTCCCCTTTCAAG-3′
	5′-CGGTCCGGCTCGAGCCAGTGGAAAGTTGTAGTAGTCGCGACT-3′
ARII2a:	5′-ATCGAAGGTCGTGGGATCCCTGCTGAGTATTCCCCTTTCAAG-3′
	5′-ATGAGGAAGCGGCGGCTCGAGGGTGACCCAGAAC-3′
ARII3a:	5′-ATCGAAGGTCGTGGGATCCCTGCTGAGTATTCCCCTTTCAAG-3′
	5′-TCACCATGCCTCGAGGGTACCACACATCAGGTGCGGTGAAG-3′
ARII4a:	5′-ATCGAAGGTCGTGGGATCCCTGCTGAGTATTCCCCTTTCAAG-3′
	5′-ACACCCAGAAGCTTCATCTCGAGAGATCAGGCAGGTCTTCT-3′
ARII3a1:	5′-ATCGAAGGTCGTGGGATCCCTGCTGAGTATTCCCCTTTCAAG-3′
	5′-ACCACCACCACTCGAGTCCATACAACTGGCCTTCTTCG-3′
ARII3a2:	5′-ATCGAAGGTCGTGGGATCCCTGCTGAGTATTCCCCTTTCAAG-3′
	5′-AATATATTCCTCGAGCCTCGCCGCCGCCGC-3′
ARII3a3:	5′-ATCGAAGGTCGTGGGATCCCTGCTGAGTATTCCCCTTTCAAG-3′
	5′-ATAAATTTTCTCGAGCCCCTGAGGGGG-3′
ET-MCM7n:	5′-GGCACATATGGCACTGAAGGACTACGCGCT-3′
	5′-ACGAGGGATCCTTCCCACAGGCACCTGATC-3′
MCM7 RT-PCR	5′-TCAATTTGTGAGAATGCCAGGCGC-3′
	5′-CACAGTTACCAACTTCCCCACAGA-3′
b-actin RT-PCR	5′-TCAAGATCATTGCTCCTC CTGAGC-3′
	5′-TGCTGTCACCTTCACCGTTCCAGT-3′