Figure 5.

siRNA-mediated depletion of Ect2, Trio, or Vav3 expression suppresses migration in vitro and invasion ex vivo. Glioblastoma cells were transfected with siRNA targeting either luciferase (ctrl), Ect2, Trio, or Vav3. Cells not transfected (NT) with siRNA were also used as an additional control. A: After 24 hours, cells were plated onto 10-well glass slides precoated with glioma-derived ECM as previously described.17 Cell migration was determined for 24 hours. Data represent the mean ± SEM of three independent experiments. B: Glioblastoma cells stably expressing GFP were transfected with siRNA for 24 hours. Cells were subsequently implanted into the bilateral putamen on rat organotypic brain slices and observed after 48 hours. Depth of invasion was calculated as described in Materials and Methods. Data represent the mean (n = 4) ± SEM of the depth of invasion. Data shown are representative of two independent experiments.