Figure 7.

Depletion of Ect2 induces multinucleated cells. A: SNB19 cells were transfected with the respective siRNAs for 2 days and plated on coverslips overnight. F-actin was stained with fluorescein isothiocyanate-phallodin (green) and chromatin was stained with 4,6-diamidino-2-phenylindole (blue). Nuclei in multinucleated cells are indicated by arrowheads. B: Quantification of multinucleated cells was performed as described in Materials and Methods. Data shown are the mean ± SEM from four independent experiments.