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. Author manuscript; available in PMC: 2009 Nov 11.
Published in final edited form as: Biochemistry. 2008 Oct 16;47(45):11900–11908. doi: 10.1021/bi800981j

FIGURE 7.

FIGURE 7

Effects of genistein and etoposide on the persistence of topoisomerase IIα- and β-DNA cleavage complexes in vitro. DNA cleavage assays were performed using purified human type II enzymes. Reactions were initiated in the presence of 50 μM genistein (left panel) or etoposide (right panel). After cleavage equilibrium was established (6 min), reaction mixtures were diluted 10–fold with DNA cleavage buffer, and levels of double-stranded DNA breaks were monitored over time. Data for DNA cleavage mediated by topoisomerase IIα (hTIIα; open bars) or β (hTIIβ; closed bars) are shown. Relative levels of DNA cleavage prior to dilution were set to 100%. Error bars represent the standard deviation for three independent experiments.