Figure 6. LXR inhibits proliferation through Abcg1-dependent alteration of cholesterol homeostasis.
(A) CFSE dilution of WT T cells stimulated with pbCD3 and GW3965 in the presence of 10μg/mL LDL for 60 h. (B) Cholesterol content of plasma membrane from WT T cells revealed by fillipin staining ex vivo or after 24 h stimulation with pbCD3 and GW3965 as indicated. (C,D) CFSE dilution of purified ABCG1−/−, ABCA1−/− and control T cells stimulated with pma and ionomycin in the presence of LXR ligands for 60 h. (e) CFSE dilution of purified WT T cells stimulated with pbCD3 for 96 h in the presence of GW3965 and mevalonic acid (MVA) as indicated.