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. 2003 Dec;23(23):8637–8650. doi: 10.1128/MCB.23.23.8637-8650.2003

FIG. 3.

FIG. 3.

Notch2 protein expression in developing thymocytes. (A) CD3− CD4− CD8− gated cells (center) were further fractionated according to defined stages of early thymic development (TN1-4) for analysis of Notch2 expression within subsets (left and right). (B) Total thymus was stained with anti-CD4 and anti-CD8 for analysis of DP and SP populations. (C) Total thymus was stained with anti-CD4, anti-CD8, and either anti-TCR or anti-HSA to delineate immature from mature CD8SP. Each value is representative of the results of at least three independent experiments with two mice per analysis.