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Low level of N2IC skews thymocyte development to the CD8 lineage. (A) N2IClo thymus analyzed at 4- to 6-weeks PT. N2IClo and control thymus were stained with anti-CD4 and anti-CD8.(B) Data compiled from two independent experiments (n = 8) showing the frequencies of DP and SP populations in N2IClo (▪) and control mice (○). (C) Expression of TCR and HSA on GFP+ CD8+ gated thymocytes from N2IClo and control thymus stained with anti-CD4, anti-CD8, and either anti-TCR or anti-HSA.
