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. 2009 Jan 15;20(2):616–630. doi: 10.1091/mbc.E08-06-0628

Figure 3.

Figure 3.

An-Nup37 and An-ELYS are stable NPC core components and are also present on SPBs during mitosis. (A) Endogenously GFP-Tagged An-Nup37 and An-ELYS cells were fixed and stained with DAPI to visualize DNA. A single nonconfocal image is shown. (B–F) The location of endogenously GFP-Tagged Nups as well as SPB and kinetochore markers were followed using live cell spinning disk confocal microscopy. (B) An-ELYS and An-Nup120 colocalize at the NPCs during mitosis. See Supplemental Video 1. (C) An-ELYS remains at NPCs during mitosis when peripheral Nups such as An-Nup49 are dispersed (Supplemental Video 2). (D and E) During mitosis, An-ELYS does not locate to the kinetochores (An-Ndc80) but accumulates at the SPBs (An-Gcp3), which are indicated by arrowheads (Supplemental Video 3). (F) SonBcNup96, An-Nup37, and An-Ndc1 (Supplemental Videos 5–7) remain at the NPCs throughout the cell cycle and also transiently locate to the SPBs during mitosis (indicated by arrowheads). Bar, 5 μm.