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. 2009 Jan 15;20(2):631–641. doi: 10.1091/mbc.E08-01-0099

Figure 1.

Figure 1.

Schematic representation of the constructs used in this study. (A) Telomeric repeats are represented as solid arrowheads; the core X as gray rectangle; ARS1 as dotted rectangle; STAR, URA3, TRP1, and the portion of ADH4 used for targeted integration in the VII-L telomere as open rectangles. Arrows above TRP1 and URA3 indicate the orientation of the gene. ACS (solid diamond), B1 (solid triangle), B2 (inverted open triangle), and Abf1 binding site (open circle) are depicted within ARS1 and core X. The Gal4 binding site in GF100 and URA-UASGAL-tel is shown as an open rectangle. (B) The consensus B1/ACS sequence (solid rectangle and solid triangle indicate the positions of B1 and ACS) is shown on top. The HMR-E, core X and ARS1 sequences encompassing B1/ACS are shown in capital letters with the consensus bases shown in bold. The mutated sequences in the ACS− and B1− constructs are shown in small letters and are underlined.