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MCM5 has poor insulating activity. W303 cells (A) and W303Δrif1 (B) cells harboring the GF100 reporter (shown on the top) in the VII-L telomere were transformed with plasmids carrying expression cassettes for the GDB-fusion proteins shown on the left. %FOAR, %TRP+, and %FOAR&TRP+ cells for each recombinant protein were measured and plotted in the top, middle, and bottom graphs, respectively. Raw data are shown in Supplemental Table 6.
