FIG. 2.

Inhibition of hematopoietic differentiation by Lnk in cultured AGM cells. (A) E11.5 AGM cells were cultured with SCF (100 ng/ml), bFGF (1 ng/ml), and OSM (10 ng/ml). On day 2 of culture, cells were infected with GFP and/or Lnk retrovirus. On day 7 of culture, adherent cells in the vehicle- and Lnk-infected AGM cultures showed normal proliferation, and no significant difference in the number of the GFP⁺ adherent cells between these cultures was observed. (B) On day 7 of culture, nonadherent cells were stained with anti-CD45 antibody, and 10⁴ cells were analyzed by flow cytometry. Representative plots of four independent experiments are shown. (C) The percentage of the CD45⁺ cells in the GFP⁺ cells was determined. Error bars indicate the standard error of the mean (n = 4).