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. 2003 Dec;23(23):8486–8494. doi: 10.1128/MCB.23.23.8486-8494.2003

FIG. 5.

FIG. 5.

Requirement of the SH2 domain of Lnk for interaction with c-Kit in cultured AGM cells. (A and B) Cultured AGM cells were infected with retroviruses encoding either Flag-tagged Lnk or Lnk-R364E together with a retrovirus encoding c-Kit. The cells were stimulated with SCF (100 ng/ml) for 20 min. Cell extracts were subjected to immunoprecipitation with anti-Flag antibody (A) and anti-c-Kit antibody (B). Precipitates or lysates were separated by SDS-PAGE and analyzed by immunoblotting with anti-c-Kit antibody (A) and anti-Flag antibody (B). The expression of Lnk and c-Kit was monitored.