TABLE 1.
Colony-forming activities in AGM-derived nonadherent cells in culturea
| Cell source for colony assay | Infection | No. of colonies per 105 nonadherent cells
|
||||
|---|---|---|---|---|---|---|
| CFU-G | CFU-M | CFU-GM | CFU-E | CFU-Mix | ||
| GFP+ cells | Mock | 9.8 ± 5.3 | 58.0 ± 2.3 | 1.8 ± 1.0 | 0.0 ± 0.0 | 0.7 ± 0.3 |
| Lnk | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | |
| GFP+/− cells | Mock | 85.9 ± 7.2 | 605.3 ± 8.9 | 16.8 ± 2.3 | 11.3 ± 4.5 | 8.5 ± 3.3 |
| Lnk | 109.0 ± 7.2 | 699.3 ± 10.0 | 20.3 ± 1.8 | 5.5 ± 6.1 | 5.5 ± 2.8 | |
a
Nonadherent cells expanded in the retrovirus-infected AGM cultures were inoculated in methylcellulose medium containing SCF (100 ng/ml), IL-3 (20 ng/ml), and EPO (4U/ml). Colonies were scored at 7 days of culture. Colony types: G, granulocyte; M, macrophage; GM, granulocyte and macrophage; E, erythrocyte; Mix, graneulocyte, macrophage, erythrocyte, and megakaryocyte. Results represent the mean ± standard error of triplicate samples.