Table 3.
Kinetic characterization of MurF inhibition by MurFp1 with respect to D-Ala-D-Ala.
| Kinetic parameters | [MurFp1] (μM) | Value |
|---|---|---|
| IC50 (μM) | 250 ± 10 | |
| Vmax (nmol/min) | 0 | 0.61 ± 0.01 |
| 75 | 0.49 ± 0.01 | |
| 150 | 0.4 ± 0.01 | |
| 300 | 0.36 ± 0.02 | |
| 600 | 0.22 ± 0.01 | |
| Km (μM) | 0 | 115 ± 10 |
| 75 | 100 ± 10 | |
| 150 | 85 ± 5 | |
| 300 | 140 ± 30 | |
| 600 | 115 ± 25 | |
| Mixed α | 0.85 ± 0.25 | |
| Mixed β | 8.5 × 10-10 ± 0.6 × 10-10 | |
| Mixed Ki (μM) | 420 ± 100 | |
| Non-competitive β | 8 × 10-10 ± 0.7 × 10-10 | |
| Non-competitive Ki (μM) | 370 ± 55 |
Kinetic parameters were monitored with a pre-incubation of 30 min with MurF, MurFp1 and UDP-MurNac-Ala-Glu-meso-A2pm.